Terial microbiota over time in this animal. (EPS)AcknowledgmentsThe Primate Services Unit at the CNPRC and Zhong-Min Ma and Tracy Rourke provided excellent technical assistance.Author Thiazole Orange custom synthesis ContributionsConceived and designed the experiments: GS PG CM. Performed the experiments: KR LF GS. Analyzed the data: KR GS CM PG. Wrote the paper: CM GS PG.Supporting InformationFigure S1 Principal 
Coordinate Analysis of Macaque Microbiota. Each macaque is represented by one type of symbol and there
Aspergillus fumigatus is the commonest etiologic agent of various clinical forms of bronchopulmonary aspergillosis including allergic, acute invasive and chronic pulmonary aspergillosis (CPA). The disease has a global distribution and it is widespread in India [1]. Invasive aspergillosis is the most severe manifestation with an overall annual incidence varying from 2 to 10 in the immunosuppressed patient population whereas CPA affects primarily immunocompetent individuals with an estimated prevalence of 3 million worldwide [2,3]. Azoles, such as itraconazole, voriconazole, and posaconazole are among the recommendedfirst-line drugs in the treatment and prophylaxis of aspergillosis [4,5]. Azole resistance is an emerging problem in A. fumigatus in Europe and has been shown to be associated with increased probability of treatment failure [6?]. Azole resistance is commonly due to mutations in the cyp51A gene, which encodes 14-a-demethylase in the ergosterol biosynthesis pathway. In azoleresistant clinical A. fumigatus isolates a wide variety of mutations in the cyp51A gene have been found, such as substitutions at codons G54, G138, P216, F219, M220 and G448 [9?2]. However, in the Netherlands a different resistance mechanism consisting of the L98H substitution, together with a 34-bp tandem repeat (TR34) in the promoter region 23977191 of this gene (TR34/L98H) was found to beAzole Resistant A. fumigatus from Indiapresent in over 90 of azole resistant isolates [13]. The TR34/ L98H resistance mechanism has been endemic in the Netherlands and subsequently reported from other European countries such as Denmark, France, Germany, Spain and the United Kingdom [12,14?9]. Isolates of A. fumigatus with TR34/L98H mutations exhibit a pan-azole resistant phenotype and were recovered primarily from azole-naive patients and from environmental sources 23727046 in the Netherlands and Denmark [15,17,20,21]. These observations suggest that patients acquire azole-resistant Aspergillus from environmental sources rather than arising through azole therapy. The consequence 
of this type of resistance development is that patients at risk can be exposed to and infected by azole-resistant strains in the environment. Furthermore, TR34/L98H isolates were cross-resistant to certain azole fungicides employed extensively in agriculture for crop protection against phytopathogenic molds, to prevent post-harvest spoilage [21]. An environmental route of resistance development poses a major challenge because multiplication and spread of resistant strains in the Pleuromutilin web environment can be anticipated. Recently, we reported from India the occurrence of TR34/L98H mutations in the cyp51A gene in A. fumigatus isolates from patients with chronic respiratory disease who had not previously been exposed to azoles [22]. This emergence of resistance in Indian clinical isolates prompted us to undertake a wide environmental survey of azole resistant A. fumigatus isolates in India. Herein, we report multi-triazole resistant environmental A.Terial microbiota over time in this animal. (EPS)AcknowledgmentsThe Primate Services Unit at the CNPRC and Zhong-Min Ma and Tracy Rourke provided excellent technical assistance.Author ContributionsConceived and designed the experiments: GS PG CM. Performed the experiments: KR LF GS. Analyzed the data: KR GS CM PG. Wrote the paper: CM GS PG.Supporting InformationFigure S1 Principal Coordinate Analysis of Macaque Microbiota. Each macaque is represented by one type of symbol and there
Aspergillus fumigatus is the commonest etiologic agent of various clinical forms of bronchopulmonary aspergillosis including allergic, acute invasive and chronic pulmonary aspergillosis (CPA). The disease has a global distribution and it is widespread in India [1]. Invasive aspergillosis is the most severe manifestation with an overall annual incidence varying from 2 to 10 in the immunosuppressed patient population whereas CPA affects primarily immunocompetent individuals with an estimated prevalence of 3 million worldwide [2,3]. Azoles, such as itraconazole, voriconazole, and posaconazole are among the recommendedfirst-line drugs in the treatment and prophylaxis of aspergillosis [4,5]. Azole resistance is an emerging problem in A. fumigatus in Europe and has been shown to be associated with increased probability of treatment failure [6?]. Azole resistance is commonly due to mutations in the cyp51A gene, which encodes 14-a-demethylase in the ergosterol biosynthesis pathway. In azoleresistant clinical A. fumigatus isolates a wide variety of mutations in the cyp51A gene have been found, such as substitutions at codons G54, G138, P216, F219, M220 and G448 [9?2]. However, in the Netherlands a different resistance mechanism consisting of the L98H substitution, together with a 34-bp tandem repeat (TR34) in the promoter region 23977191 of this gene (TR34/L98H) was found to beAzole Resistant A. fumigatus from Indiapresent in over 90 of azole resistant isolates [13]. The TR34/ L98H resistance mechanism has been endemic in the Netherlands and subsequently reported from other European countries such as Denmark, France, Germany, Spain and the United Kingdom [12,14?9]. Isolates of A. fumigatus with TR34/L98H mutations exhibit a pan-azole resistant phenotype and were recovered primarily from azole-naive patients and from environmental sources 23727046 in the Netherlands and Denmark [15,17,20,21]. These observations suggest that patients acquire azole-resistant Aspergillus from environmental sources rather than arising through azole therapy. The consequence of this type of resistance development is that patients at risk can be exposed to and infected by azole-resistant strains in the environment. Furthermore, TR34/L98H isolates were cross-resistant to certain azole fungicides employed extensively in agriculture for crop protection against phytopathogenic molds, to prevent post-harvest spoilage [21]. An environmental route of resistance development poses a major challenge because multiplication and spread of resistant strains in the environment can be anticipated. Recently, we reported from India the occurrence of TR34/L98H mutations in the cyp51A gene in A. fumigatus isolates from patients with chronic respiratory disease who had not previously been exposed to azoles [22]. This emergence of resistance in Indian clinical isolates prompted us to undertake a wide environmental survey of azole resistant A. fumigatus isolates in India. Herein, we report multi-triazole resistant environmental A.
