Product Name :
LC3B monoclonal antibody (2G6)
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Stable at -20°C up to 1 year.
Description:
Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus.{{1702967-37-0} web|{1702967-37-0} Purity & Documentation|{1702967-37-0} Formula|{1702967-37-0} supplier} Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.{{2568868-35-7} medchemexpress|{2568868-35-7} Protocol|{2568868-35-7} Purity|{2568868-35-7} custom synthesis} Figure: Western blot analysis of endogenous LC3 using MAb to LC3B .PMID:29763120 Method: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal Antibody to LC3 (2G6) (0.5 µg/ ml) for 1h at RT and developed by ECL (exposure time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT Lane 8: Neuro2A Lane 9: NIH – 3T Figure: Western blot analysis of endogenous LC3 using MAb to LC3B .Method: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal Antibody to LC3 (2G6) (0.5 µg/ ml) for 1h at RT and developed by ECL (exposure time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT Lane 8: Neuro2A Lane 9: NIH – 3T
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| Alternative Name Microtubule-associated protein 1 light chain 3B, MAP1LC3B | Application ICC, WB | Application Notes Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot. | Clone 2G6 | Formulation Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose. | Host Mouse | Immunogen Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin. | Isotype IgG1 | Positive Control Included. | Recommendation Dilutions/Conditions Immunocytochemistry (paraformaldehyde/methanol fixed cells; 1-10 µg/ml)Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)We strongly recommend to use PVDF membranes for immunoblot analysis.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Hamster, Human, Monkey, Mouse, Rat | Specificity Recognizes both forms of endogenous LC3B. | UniProt ID Q9GZQ8 | Unit of Measure (UM) µg
