ophilia evaluation and anticoagulation management. Strategies: A total of 438 physicians and sophisticated practice providers (APPs) have been surveyed with a questionnaire composed of both subjective and objective things related to their information and practice expertise with anticoagulation and thrombophilia. 404 in the 438 surveys have been fully completed and analyzed utilizing a combination of COX-2 Activator manufacturer chi-square, Kruskal-Wallis with post-Hoc Dunn test, unpaired T-test and test item analysis exactly where acceptable. Benefits: The general median doctor score of 61.three was significantly higher compared to APPs score of 52.4 (P = 0.001). Among specialties for the physicians, the Hospitalist and cardiology sub-groups had drastically larger median score in comparison to various medical specialties (table 1). The information differential between physician and APP was not substantial at 0 years of encounter but was present at four years of encounter (P = 0.05).FIGURE 1 Doctor Vs APP Total ScoreFIGURE two Scores of Physician Respondents according to Specialty Conclusions: Our study shows a considerable knowledge gap among providers of varying degrees and providers within diverse subspecialties. This may perhaps permit for any specific target audience for increased education concerning aspects of anticoagulation management and thrombophilia evaluation within the future.PB1163|C20209T Prothrombin Gene Mutation in a Caucasian Woman having a History of Venous Thrombosis D. Jambou1; N. Saut 2; V. Queyrel-Moranne3; A. Appert-Flory1; F. Fischer1; P. Suchon2; P. ToulonCote d’Azur University, Pasteur University Hospital, Hematology,Good, France; 2Assistance Publique Hopitaux de Marseille, Hematology, Marseille, France; 3Cote d’Azur University, Pasteur University Hospital, Rheumatology, Nice, France Background: Whereas the G20210A (c.97GA) prothrombin gene variant has been related with an elevated danger of venous thromboembolism (VTE), other uncommon mutations in F2 gene have been reported, with unknown frequency and potential association with VTE. Aims: To report a case having a uncommon F2 mutation. Techniques: A 69 years old Caucasian woman presented with an unprovoked deep venous thrombosis with the leg. The thrombophilia workup incorporated common coagulation tests, antithrombin, protein C, protein S, anticardiolipin and antibeta2GP1 antibodies, (isotypes G and M), lupus anticoagulant (LA) and F2 and F5 gene polymorphismsABSTRACT853 of|determination by melting curve analysis following real-time PCR on a capillary-based Lightcycler utilizing an ABI 3500XL. Results: Antithrombin, proteins C/S, anticardiolipin and antibeta2GP1B were inside their normal ranges. LA was unfavorable and F5 genotyping for G1691A transition revealed a wild-type genotype. By contrast, melting curve evaluation for G20210A polymorphism showed an unusual curve progression (figure 1), with Tm (57.5 and 63.5 ), whereas the melting pointsTM corresponding to the wild-type and also the 20210 A allele were defined by the manufacturer to become between 617 and 516 , respectively. Sequencing revealed a CT CDK6 Inhibitor Compound heterozygous transition at position 20209. The retrospective evaluation of test final results obtained in our two hospitals amongst 2007 and 2018, revealed 21 heterozygous carriers in the C20209T mutation among 26.000 determinations (incidence: 0.08 ).TMAims: Description of a heterozygote FVL having a catastrophic clinical presentation. Techniques: Collection of clinical data in SCl icoapplication. Outcomes: Lady, 26 years old, with overweight and estrogencontaining oral contracep
