Ich, Germany). The membranes have been blotted with ten skim milk, washed with Tris-buffered

Ich, Germany). The membranes have been blotted with ten skim milk, washed with Tris-buffered CD360/IL-21R Proteins custom synthesis saline (20 mM Tris HCL, 137mM NaCl) with 0.1 Tween 20 then probed with appropriate antibodies (Egr-1, MIP-2, actin and histone: Santa Cruz Biotechnology, Santa Cruz, CA; A20: Oncogene Study Items, San Diego, CA; HO-1: Stressgen Biotechnologies Corporation, San Diego, CA). Just after washing in Tris-buffered saline containing 0.1 Tween 20, the membrane was incubated withhorseradish peroxidase-conjugated secondary antibody (Amersham, Tiny Chalfont, UK) for another 1 hour, after which visualized by enhanced chemiluminescence according to the manufacturer’s suggestions (Amersham).Intracellular Expression of ET-1, HO-1, A20, Interleukin-10 (IL-10), Inducible Nitric Oxide Synthase (iNOS), and CXCR2 by ImmunostainingThe paraffin sections of the liver biopsies have been immunochemically stained for ET-1, HO-1, A20, iNOS, and CXCR2 working with Dako EnVision system (Dako, Glostrup, Denmark). In brief, after de-paraffinization, endogenous peroxidase activity was quenched by immersing the sections for 30 minutes in absolute methanol containing 0.three H2O2. The sections had been processed to unmask the antigens by standard microwave oven heating in ten mol/L citric acid bufferFIGURE 1. Time course with the (a) mean arterial pressure and (b) Share this post on: