Product Name :
Shrimp alkaline phosphatase, (recombinant)
Sequence:
Purity:
Molecular Weight:
54 kDa
Solubility :
Appearance:
Use/Stability :
Stable for 2 years at -20°C, for 6 months at 4°C, or for 3 months at 25°C
Description:
Heat-labile alkaline phosphatase for dephosphorylation of DNA, RNA and nucleotides Very high specific activity Completely heat-inactivated at 65°C Removes 5’-phosphates from DNA, RNA, dNTPs and proteins Works in restriction enzyme buffers Used in PCR clean-up to treat dNTPs prior to DNA sequencing or SNP analysis Shrimp alkaline phosphatase (recombinant), (rSAP) is a multipurpose alkaline phosphatase that is fully inactivated by a short heat treatment.70989-04-7 MedChemExpress rSAP has all the properties of the native enzyme, but with the additional benefits of greater stability, consistency, and purity.1313883-00-9 web rSAP simplifies cloning and sequencing workflows due to a complete heat inactivation.PMID:28121117 Fig 2. Stability of Shrimp alkaline phosphatase at room temperature Fig 3. Cloning workflow using Shrimp alkaline phosphatase (SAP) Fig 4. Sequencing workflow using Shrimp alkaline phosphatase (SAP) Fig 1. Heat inactivation of Shrimp alkaline phosphatase at 65°C and 75°C Fig 2. Stability of Shrimp alkaline phosphatase at room temperature Fig 3. Cloning workflow using Shrimp alkaline phosphatase (SAP) Fig 4. Sequencing workflow using Shrimp alkaline phosphatase (SAP) Fig 1. Heat inactivation of Shrimp alkaline phosphatase at 65°C and 75°C
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Solubility:
Formula:
Additional Information :
| Alternative Name rSAP | Concentration 1U/µl for 1000 unit size5U/µl for 5000 unit size | Formulation Liquid. | MW 54 kDa | Purity Detail Purified by SDS-PAGE. DNase, RNase or protease activities not detected. | Source Pandalus borealis (Northern red shrimp) origin, recombinantly produced in Pichia pastoris. | Specific Activity >2000 units/mg. One unit of Shrimp alkaline phosphatase (recombinant) converts 1µmol p-nitrophenyl phosphate per minute to nitrophenol and phosphate at 37°C and pH 10.4 in 0.1 M glycine buffer, 1 mM each of ZnCl2 and MgCl2 and 6 mM 4-nitrophenyl phosphate. | UniProt ID Q9BHT8_PANBO
