GST Nanoselector Magnetic beads

Description:
| Description: GST Nanoselector Magnetic beads have been specifically designed to bind GST-fusion proteins. GST Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. GST Nanoselector Magnetic beads are ideal tools to isolate or purify GST-fusion proteins fast and efficiently.Ligand: Anti-GST single domain antibody fragment (VHH, Nanobody) Bead size: ~ 2.8µmReactivity: Recognizes GST fusion protein selectivelyBinding | Capacity: High binding capacity, 10 µL slurry bind about 25 µg of recombinant GST. | Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.Storage | Buffer: 50 % slurry in PBS containing 20 % Ethanol | Background:Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification.{{897326-30-6} site|{897326-30-6} Biological Activity|{897326-30-6} References|{897326-30-6} custom synthesis} GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay.{{2641998-63-0} MedChemExpress|{2641998-63-0} Protocol|{2641998-63-0} In Vivo|{2641998-63-0} custom synthesis} This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest.PMID:30969616 Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein. GST Nanoselector Agarose is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.