Product Name :
Anti-His tag, Llama antibody
Applications:
WB,ELISA,IP,Flow Cyt
Reactivity :
His tag
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-His tag, Llama antibody is designed for detecting His tag fusion proteins specifically. Anti-His tag, Llama antibody is recombinant fab of alpaca IgG1 fused to Llama IgG Fc. Based on western blot and ELISA, Anti-His tag, Llama antibody detects the His tag selectively, no reactivity with other proteins. | Immunogen: 6*His tag fusion protein | Host: Alpaca pacous | Isotype: Fab of alpaca IgG1 fused to Llama IgG Fc | Conjugate: Unconjugated | Specificity: His tag sequence(HHHHHH) | Cross-Reactivity: Highly selective for His tag sequence | Purity: Recombinant expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles) | Background:The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Description2 :
Anti-His tag, Llama antibody is designed for detecting His tag fusion proteins specifically. Anti-His tag, Llama antibody is recombinant fab of alpaca IgG1 fused to Llama IgG Fc. Based on western blot and ELISA, Anti-His tag, Llama antibody detects the His tag selectively, no reactivity with other proteins.
Immunogen:
6*His tag fusion protein
Host :
Alpaca pacous
Isotype:
Fab of alpaca IgG1 fused to Llama IgG Fc
Purity :
Recombinant expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles)
Function:
WB: 1:5000-1:20000 ELISA: 1:5000-1:20000 IP: 2ug/sampleFlow Cyt： 1:200-1:2000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc.{{2704316-82-3} MedChemExpress|{2704316-82-3} Biological Activity|{2704316-82-3} Formula|{2704316-82-3} custom synthesis} The actual dilution used must be determined empirically.{{1189971-04-7} MedChemExpress|{1189971-04-7} Biological Activity|{1189971-04-7} In Vivo|{1189971-04-7} supplier}
