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(2021) 22:899 Sun et al. BMC Genomics doi.org/10.1186/s12864-021-08213-wRESEARCHOpen AccessTranscriptome comparative evaluation of ovarian follicles reveals the key genes and Nav1.2 list signaling pathways implicated in hen egg productionXue Sun1,2, Xiaoxia Chen1,two, Jinghua Zhao1,2, Chang Ma1,two, Chunchi Yan1,2, Simushi Liswaniso1,two, Rifu Xu1,2 and Ning Qin1,2Abstract Background: Ovarian follicle improvement plays a crucial function in determination of poultry egg production. The follicles in the many developmental stages possess their own distinct molecular genetic qualities and have various biological roles in chicken ovary development and function. In the every stage, many genes of follicle-specific expression and biological pathways are involved inside the vary-sized follicular development and physiological events. Identification from the pivotal genes and signaling pathways that manage the follicular improvement is beneficial for understanding their exact regulatory functions and molecular mechanisms underlying egg-laying traits of laying hens. Outcomes: The comparative mRNA transcriptomic evaluation of ovarian follicles at three crucial developmental stages such as slow growing white follicles (GWF), compact yellow follicles (SYF) of recruitment into the hierarchy, and differentiated large yellow follicles (LYF), was achieved in the layers with decrease and greater egg production. Entirely, 137, 447, and 229 of up-regulated differentially expressed genes (DEGs), and 99, 97, and 157 of down-regulated DEGs within the GWF, SYF and LYF follicles, which includes VIPR1, VIPR2, ADRB2, and HSD17B1 had been identified, respectively. Additionally, NDUFAB1 and GABRA1 genes, two most promising candidates potentially linked with egg-laying performance have been screened out from the 13 co-expressed DEGs within the GWF, SYF and LYF samples. We additional investigated the biological effects of NDUFAB1 and GABRA1 on ovarian follicular development and located that NDUFAB1 promotes follicle improvement by stimulating granulosa cell (GC) 5-HT Receptor Antagonist custom synthesis proliferation and decreasing cell apoptosis, increases the expression of CCND1 and BCL-2 but attenuates the expression of caspase-3, and facilitates steroidogenesis by enhancing the expression of STAR and CYP11A1. In contrast, GABRA1 inhibits GC proliferation and stimulates cell apoptosis, decreases the expression of CCND1, BCL-2, STAR, and CYP11A1 but elevates the expression of caspase-3. Moreover, the 3 important signaling pathways like PPAR signaling pathway, cAMP signaling pathway and neuroactive ligand-receptor interaction had been drastically enriched, which may play necessary roles in ovarian follicle development, differentiation, follicle selection, and maturation.Correspondence: [email protected]; [email protected] Xue Sun and Xiaoxia Chen contributed equally to this perform. 2 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technologies, Jilin Agricultural University, Changchun 130118, China Full list of author facts is readily available at the finish of the articleThe Author(s) 2021. Open Access This short article is licensed under a Creative Commons Attribution four.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give proper credit towards the original author(s) and also the supply, deliver a link towards the Creative Commons licence, and indicate if adjustments have been created. The pictures or
