Pared with the other 3 comparisons (Supplementary Figure S7), indicating that these DEGs promoted floral transition in L. gratissima.Co-expression Module Analysis for DEGsWGCNA is really a systems biology process for analyzing the correlation relationships amongst genes in multiple samples (LangfelderAugust 2021 | Volume 12 | ArticleFrontiers in Plant Caspase Inhibitor manufacturer Science | www.frontiersin.orgLiu et al.Photoperiod-Induced Floral Transition of Luculia gratissimaand Horvath, 2008). Within this study, the outcomes of WGCNA showed that 1,226 DEGs in eight samples have been clustered in 11 different co-expression modules (labeled with unique colors; Figure 4A). It can be noteworthy that 4 out of 11 co-expression modules substantially correlated with a single sample (r 0.9, p 0.05; Figure 4B and Supplementary Table S6). For instance, the largest module (black module) included 247 (20.15 ) SD19-specific DEGs (Figure 4B and Supplementary Table S6A). We further carried out GO enrichment analysis on 11 co-expression modules, and only the greenyellow module was not drastically enriched for any GO terms (Supplementary Table S7). Some GO terms were especially identified in only a single module. By way of example, 120 distinct GO terms have been identified inside the black module, which primarily involved signal transduction and negative regulation of metabolic processes, and 34 module-specific GO terms were identified within the brown module, which was mainly connected with development and improvement (Supplementary Table S7). Nevertheless, a number of GO terms, such as “response to organic substance” and “response to a stimulus,” appeared in various modules (Supplementary Table S7), indicating attainable module-gene interactions. Overall, the extensively enriched GO terms showed that several biological processes were involved in the floral transition in L. gratissima. The 11 modules had been divided into seven categories depending on the correlations among modules (Figure 4C). The heat map showed that there was a high correlation between the blue, magenta, pink, and tan modules, in which the genes were highly expressed in SD7 and SD10 (Figures 4B,C), and were drastically enriched in many GO terms involving secondary metabolite biosynthesis, signal transduction, and regulation of developmental processes (Supplementary Table S7).signal-related genes have been identified, involving 23 sugar signalrelated homologs. These genes expressed differently in distinct improvement stages of L. gratissima. By way of example, HDAC11 Inhibitor medchemexpress HEXOKINASE (HK) homologs (Unigene0044869 and Unigene0044870) have been significantly upregulated in SD7-vs.-LD7 and SD13-vs.-LD13, and a BETA-GLUCOSIDASE 24 homolog (Unigene0013088) was drastically upregulated in SD10-vs.-LD10. Meanwhile, Unigene0009721 and Unigene0041893, homologs of GALACTINOL SYNTHASE 2 and RAFFINOSE SYNTHASE participating in raffinose synthesis, have been upregulated in SD7-vs.-LD7. Furthermore, TREHALOSE-6-PHOSPHATE SYNTHSE (TPS) homologs (Unigene0019787, Unigene0024389, Unigene0013555, Unigene0054604, Unigene0004913, and Unigene0062998) were upregulated at various stages, and SWEET16 homolog (Unigene0012661) was drastically upregulated in SD7-vs.-LD7 and SD10-vs.-LD10 (Figure 5E and Supplementary Table S9). Hence, these genes may straight or indirectly participate in floral transition in L. gratissima.Identification of DEG Expression Patterns Related With Floral Transition in L. gratissimaAccording to the above functional classifications and WGCNA of these DEGs, and flowering-rel.
