Bars, 50 m. (F) The mRNA levels of inflammation (TNF-, IL-1, and IL-6) in MAECs of mice (n = eight). The information are presented because the means SEM. P 0.05 versus NCD-WT, P 0.01 versus NCD-WT, P 0.001 versus NCD-WT; P 0.05 versus WD-WT, P 0.01 versus WD-WT, P 0.001 versus WD-WT Meng et al., Sci. Adv. 2021; 7 : eabe6903 21 May well 2021 3 ofSCIENCE ADVANCES Research ARTICLEFig. 2. Myeloid cell pecific MYDGF deficiency is associated with atherosclerotic plaque formation in AKO mice. AKO and DKO mice aged four to 6 weeks have been fed a WD for 12 weeks (10 mice in every group). (A and B) The vasodilatation STAT6 manufacturer reaction induced by Ach (A) and SNP (B) (n = 10). (C) Representative pictures of en face atherosclerotic lesions. (D) Quantitative analysis of (C) (n = 5). (E) Representative pictures on the cross-sectional area from the aortic root (n = eight). Scale bars, 500 m. (F) Quantitative evaluation of (E). (G) Representative immunohistochemical staining images of VSMCs [ mooth muscle actin (-SMA)], collagen (Masson), macrophages (anti-CD68), and T lymphocytes (anti-CD3) in aortic plaques. Scale bar, one hundred m. (H) Quantitative evaluation of (G) (n = 8). (I and J) The mRNA levels of adhesion molecules (VCAM-1, ICAM-1, and E-selectin) (I) and inflammation (TNF-, IL-1, and IL-6) (J) in MAECs of mice (n = five). The data are presented because the suggests SEM. P 0.05 and P 0.001. Meng et al., Sci. Adv. 2021; 7 : eabe6903 21 Could 2021 4 ofSCIENCE ADVANCES Research ARTICLEFig. 3. BMT alleviated endothelial injury and atherosclerosis in mice. As shown in fig. S4C, BMT was performed, and atherosclerosis was assessed right after WD feeding for 12 weeks (10 mice in every single group). (A) The aortic vasodilatation induced by Ach in KO mice (n = 10). (B) Representative photos of TUNEL staining in sections of thoracic aortas. Scale bars, 200 m. (C) The percentage of apoptotic endothelial cells (n = five). (D) Representative electron microscopy photos of endothelium in KO mice (n = five). Scale bars, 50 m. (E) Representative images of en face atherosclerotic lesion locations in AKO and DKO mice. (F) Quantitative evaluation of (E) (n = five). (G) Representative photos of the cross-sectional area on the aortic root in AKO and DKO mice. Scale bars, 500 m. (H) Quantitative evaluation of (G) (n = 8). (I) Representative immunohistochemical staining pictures of VSMCs, collagen, macrophages, and T lymphocytes in aortic plaques. Scale bar, 100 m. (J) Quantitative evaluation of (I) (n = five). The data are presented as the 5-HT5 Receptor Agonist custom synthesis implies SEM. P 0.05 versus WT WT and P 0.01 versus WT WT; #P 0.05 versus WT KO and ##P 0.001 versus WT KO; P 0.01 versus WT AKO; P 0.001 versus WT DKO. Meng et al., Sci. Adv. 2021; 7 : eabe6903 21 Could 2021 five ofSCIENCE ADVANCES Study ARTICLEThus, KO mice received intramarrow injection of AAV-MYDGF every 3 weeks for 12 weeks, plus the results showed that plasma MYDGF was maintained at a sustained high level (fig. S6B). In parallel, bone marrow MYDGF mRNA and protein levels, also as the fluorescence expression, in AAV-MYDGF mice had been greater than these in AAV reen fluorescent protein (GFP) mice at 12 weeks (fig. S6, C to E). Then, formal experiments including WT, KO + AAV-GFP (KO-GFP), and KO + AAV-MYDGF (KO-MYDGF) groups, as shown in fig. S6F, were performed. The outcomes showed that AAV-MYDGF improved endothelial function, decreased endothelial cell apoptosis (Fig. 4, A to D), lowered inflammation and adhesion molecule expression of MAECs, improved IR, and decreased body weight achieve (fig. S7, A to H), compared with.
