On NextSeq High Output single-end sequencing run. Outcomes: Administration of AFSC-EVs improved terminal bud density and surface location of lung explants back to handle levels and promoted lung epithelial cell differentiation in lung organoids (elevated SPC andPF12.10=OWP2.HIV-specific antibody-mediated targeting of ENV+ tissues by exosomes Zou Xue, Yuan M’eng, Zheng Nan and Wu Zhiwei Nanjing University, Nanjing, China (People’s Republic)Introduction: ART (Antiretroviral Therapy) can efficiently suppress HIV replication within the peripheral blood to an undetectable level. Having said that, efforts to eradicate the latent virus in reservoirs stay a challenge and are a major obstacle within the treatment of HIV patients. Exosomes exhibit enormous guarantee as an endogenous drug delivery nanosystem for delivering drugs to reservoir tissues offered their distinctive properties, including low immunogenicity, innate stability, high delivery efficiency and mostly importantly the ability to penetrate strong tissues resulting from their lipophilic properties. Solutions: In this study, we Fc-gamma Receptor I/CD64 Proteins Recombinant Proteins engineered and expressed the ScFv of a higher affinity HIV-specific monoclonal antibody, 10E8, on exosome surface. Exosomes from 293T cells have been loaded with curcumin by means of saponin, with efficient up to 34 . 10E8ScFv-expressing exosomes (10E8-Exo) showed hugely efficient targeting of and curcumin delivery to CHO cell that expresses a trimeric gp140 on its surface (ENV+ cells) in vitro as demonstrated by confocal imaging and flow cytometry. We showed that 10E8-Exo could correctly bind to CHO cell that expresses a trimeric gp140 on its surface. The exosomes loaded with curcumin, a chemical that was shown to kill HIV-infected cells, showed certain killing from the trimeric gp140-expressing CHO cells. In an NCG mouse model that was grafted with the tumourigenic gp140-CHO cells and created solid tissue tumours intravenously injected 10E8-Exo targeted the ENV-expressing tissues and delivered curcumin to induce a strong suppression of the ENV+ tumour growth having a low toxicity. Results: Our results demonstrated that engineered exosomes can provide anti-HIV agents to strong tissues byISEV2019 ABSTRACT BOOKspecifically targeting cells expressing viral ENV and induce cell killings. Summary/conclusion: It suggesting that such an approach might be created for eradicating virusinfected cells in tissue reservoir Funding: This study was supported by The Glycophorin-A/CD235a Proteins medchemexpress National Key Analysis and Development System of China(2016YFC1201000), Nature Science Foundation of Jiangsu Province (BY2015069-02) and National Nature Science Foundation of China (81672020). The funders had no part in study style, data collection and analysis, selection to publish, or preparation in the manuscript.JOURNAL OF EXTRACELLULAR VESICLESLate breaking- EVs and cancer Chairs: Sonia Melo; Golnaz Morad Location: Level three, Hall A 15:306:LBF01.Exosomes from LNCaP cells promote the activity of osteoblasts by means of the transfer of mir-375 Yun Yea and Su-liang Liba Prostate Cancer, Xi’an, China (People’s Republic); bCancer, Xi’an, China (People’s Republic)for Cancer Analysis, Tokyo, Japan; cCancer Proteomics Group, Cancer Precision Medicine Center, Japanese Foundation for Cancer Analysis, Tokyo, JapanIntroduction: Research have shown that exosomes influence tumour metastasis, diagnosis and remedy. It has been found that exosomal miRNAs are closely linked towards the metastatic microenvironment. On the other hand, the regulatory part of exosomes from prostate cancer (PCa) cells in.
