F our experiments were also performed in COS-7 and 293 cells (of monkey and human embryonic kidney origin, respectively), and yielded outcomes related to HUVECs (see Fig. 9), Moreover, final results consistent with our findings happen to be reported inside a number of other cell varieties, including human airway [21] and rat smooth muscle cells [31].NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptAcknowledgmentsWe are thankful to Dr. James Darnell, Jr. for kindly giving us with mStat3 wt and mutant expression vectors. Funding This study was supported by Program Project Grant HL-65608 to LCB in the Anti-Mullerian Hormone Receptor Type 2 Proteins Species National Heart, Lung and Blood Institute, and from start-up funds in the Cardiovascular Institute, University of Pittsburgh, to SNM.Biochim Biophys Acta. Author manuscript; out there in PMC 2013 Might 01.Mattagajasingh et al.Page
bioengineeringReviewBioengineering of Extracellular Vesicles: Exosome-Based Next-Generation Therapeutic Technique in CancerPriyanka Saha, Suchisnigdha Datta, Sukanya Ghosh, Anurima Samanta, Paramita Ghosh and Dona Sinha Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, India; [email protected] (P.S.); [email protected] (S.D.); [email protected] (S.G.); [email protected] (A.S.); [email protected] (P.G.) Correspondence: [email protected] or [email protected]; Tel.: ++91-33-2476-5101 (ext. 327); Fax: +91-33-2475-Abstract: Extracellular nano vesicles and exosomes hold compelling proof in intercellular communication. Exosomal intracellular signal transduction is mediated by the transfer of cargo proteins, lipids, micro (mi)RNAs, extended noncoding (lnc)RNAs, compact interfering (si)RNAs, DNA, along with other functional molecules that play a pivotal role in regulating tumor growth and metastasis. Even so, emerging research trends indicate that exosomes might be utilised as a promising tool in anticancer remedy. This review functions a majority of the bioengineering applications of fabricated exosomal cargoes. In addition, it encompasses how the manipulation and delivery of certain cargoes–noncoding RNAs (ncRNAs), recombinant proteins, immune-modulators, chemotherapeutic drugs, and also other smaller molecules–may serve as a precise therapeutic method in cancer management.Citation: Saha, P.; Datta, S.; Ghosh, S.; Samanta, A.; Ghosh, P.; Sinha, D. Bioengineering of Extracellular Vesicles: Exosome-Based Next-Generation Therapeutic Strategy in Cancer. Bioengineering 2021, 8, 139. https://doi.org/ 10.3390/bioengineering8100139 Academic Editor: Prakash Gangadaran Received: 4 September 2021 Accepted: five October 2021 Published: 10 OctoberKeywords: exosome; bioengineering; immunotherapy; exosomal cargo delivery; recombinant proteins; ncRNA; chemotherapy1. Introduction Specialized nanoparticles, exosomes, have gained considerable interest from researchers and clinicians by virtue of their intercellular communication and efficient drug delivery home [1]. Exosomes are very advantageous for therapeutic purposes as a consequence of their high stability, targetability, NIMA Related Kinase 3 Proteins MedChemExpress significantly less immunogenicity, prolonged half-life, and capacity to cross the blood rain barrier (BBB) [2]. Exosomes may possibly be modified with quite a few molecules, chemotherapeutic drugs, functional proteins, and genetic materials, which may well emerge as a prospective next-generation anti-cancer approach. Exosomes may perhaps invade physiological barriers, which were usually imp.
