Res1; Brenda Louyse Olimpia Souza Teixeira2; Lara R. Quadrado2; Aldo Tavares1; Anamelia Bocca1; Felipe Saldanha-araujo1; Octavio Franco2; Rinaldo W. Pereira1 University of Brasilia, Brasilia, Brazil; 2Catholic University of Brasilia, Brasilia, BrazilPF04.Correlation of exosomal miRNA- and anthropometric profile of an active life-style Kitti Garai1; Adam Gyebrovszki2; Emese Katai3; Tamas Nagy3; Judit E. Pongracz1; Krisztian Kvell1; Marta WilhelmBackground: Extracellular vesicles (EV) can serve as carries of cellular facts. EVs derived from dendritic cells (DC) have been shown to target other immune cells and modulate their function. EVs production by DC is induced by a diverse array of signals which includes cytokines, LPS, and antigens however the part of antimicrobial peptides, for example the human cathelicidin LL37, within this method is largely unknown. In this context, we investigate no TrkC Proteins Formulation matter whether LL-37 induces and alters DC-derived EVs profile.ISEV 2018 abstract bookMethods: Murine bone marrow-derived DCs were stimulated with LPS (as a positive handle) and diverse concentrations of LL-37. EVs have been obtained from cultured cell supernatants and purified by ultracentrifugation. Particle size distribution and concentration of EVs was measured by tunable resistive pulse sensing, and transmission electron microscopy was performed to characterize their morphology. Benefits: Our preliminary final results show that LL-37 increases the concentration of and decreases the typical size of EVs when compared with LPS. EV morphology from our samples was in accordance with the literature. Summary/Conclusion: The subsequent ongoing step could be the investigation concerning the function of LL37 induced EVs inside the immunomodulation well described to be carried out by cathelicidin. Funding: This ILT-4 Proteins medchemexpress operate was funded by Funda o de Apoio Pesquisa do Distrito Federal, CNPq, CAPES and Universidade Cat ica de Brasilia.PF04.Immunoproteomic characterization of outer membrane vesicles from high-producing actinobacillus pleuropneumoniae Fabio Antenucci1; Zofia Magnowska2; Manfred Nimtz3; Camille Roesch4; Lothar J sch3; Anders Miki Bojesen1 University of Copenhagen, K enhavn S, Denmark; 2University of Copenhagen, Copenhagen, Denmark; 3Helmholtz Centre for Infection Study, Braunschweig, Germany; 4Izon Science Ltd, Lyon, FranceBackground: Outer membrane veiscles (OMVs) are developed by the majority of Gram-negative bacteria. Due to the antigenic similarity amongst OMVs plus the bacterial outer membrane, OMVs have verified to become promising for the improvement of novel vaccines against bacterial pathogens. Within this operate we describe the immunoproteomic characterization of OMVs from Actinobacillus pleuropneumoniae (App), a Gramnegative pathogen of fantastic veterinary interest, inside the context of vaccine development. Techniques: OMVs were isolated from App MIDG2331 serotype eight wild sort and an isogenic nlpI mutant working with a modified version with the hydrostatic filtration protocol described by Musante et al.. OMVs proteins were purified by Wessel-Fl e extraction and resolved by 2D Page. Protein staining and 2D western blotting had been then made use of to determine relevant protein spots, which were excised and subjected to protein identification by MALDI peptide mapping. Outcomes: Our evaluation led to the identification of numerous virulence variables in App OMVs, such as all three Apx toxins developed by App MIDG2331 (Apx II, III and IV) and proteins involved in nutrient acquisition. A number of the proteins have been also shown for the first ti.
