Bars, 50 m. (F) The mRNA levels of inflammation (TNF-, IL-1, and IL-6) in MAECs of mice (n = eight). The information are presented as the signifies SEM. P 0.05 LAG-3/CD223 Proteins Formulation versus NCD-WT, P 0.01 versus NCD-WT, P 0.001 versus NCD-WT; P 0.05 versus WD-WT, P 0.01 versus WD-WT, P 0.001 versus WD-WT Meng et al., Sci. Adv. 2021; 7 : eabe6903 21 May perhaps 2021 three ofSCIENCE ADVANCES Analysis ARTICLEFig. 2. Myeloid cell pecific MYDGF deficiency is related with atherosclerotic plaque formation in AKO mice. AKO and DKO mice aged four to 6 weeks were fed a WD for 12 weeks (ten mice in each group). (A and B) The vasodilatation reaction induced by Ach (A) and SNP (B) (n = ten). (C) Representative images of en face atherosclerotic lesions. (D) Quantitative evaluation of (C) (n = five). (E) Representative pictures on the cross-sectional region with the aortic root (n = eight). Scale bars, 500 m. (F) Quantitative analysis of (E). (G) Representative immunohistochemical staining photos of VSMCs [ mooth muscle actin (-SMA)], collagen (Masson), macrophages (anti-CD68), and T lymphocytes (anti-CD3) in aortic plaques. Scale bar, 100 m. (H) Quantitative analysis of (G) (n = 8). (I and J) The mRNA levels of adhesion molecules (VCAM-1, ICAM-1, and E-selectin) (I) and inflammation (TNF-, IL-1, and IL-6) (J) in MAECs of mice (n = 5). The information are presented because the means SEM. P 0.05 and P 0.001. Meng et al., Sci. Adv. 2021; 7 : eabe6903 21 May 2021 4 ofSCIENCE ADVANCES Analysis ARTICLEFig. 3. BMT alleviated endothelial injury and atherosclerosis in mice. As shown in fig. S4C, BMT was performed, and atherosclerosis was assessed just after WD feeding for 12 weeks (10 mice in every single group). (A) The aortic vasodilatation induced by Ach in KO mice (n = ten). (B) Representative pictures of TUNEL staining in sections of thoracic aortas. Scale bars, 200 m. (C) The percentage of apoptotic endothelial cells (n = five). (D) Representative electron microscopy images of endothelium in KO mice (n = five). Scale bars, 50 m. (E) Representative photos of en face atherosclerotic lesion regions in AKO and DKO mice. (F) Quantitative evaluation of (E) (n = five). (G) Representative pictures in the cross-sectional region from the aortic root in AKO and DKO mice. Scale bars, 500 m. (H) Quantitative evaluation of (G) (n = 8). (I) Representative immunohistochemical staining photos of VSMCs, collagen, macrophages, and T lymphocytes in aortic plaques. Scale bar, one hundred m. (J) Quantitative evaluation of (I) (n = five). The data are presented because the indicates SEM. P 0.05 versus WT WT and P 0.01 versus WT WT; #P 0.05 versus WT KO and ##P 0.001 versus WT KO; P 0.01 versus WT AKO; P 0.001 versus WT DKO. Meng et al., Sci. Adv. 2021; 7 : eabe6903 21 May 2021 five ofSCIENCE ADVANCES Analysis ARTICLEThus, KO mice received intramarrow injection of AAV-MYDGF every three weeks for 12 weeks, along with the results showed that plasma MYDGF was maintained at a sustained high level (fig. S6B). In parallel, bone marrow MYDGF mRNA and protein levels, at the same time because the fluorescence expression, in AAV-MYDGF mice had been greater than these in AAV reen fluorescent protein (GFP) mice at 12 weeks (fig. S6, C to E). Then, formal experiments such as WT, KO + AAV-GFP (KO-GFP), and KO + AAV-MYDGF (KO-MYDGF) groups, as shown in fig. S6F, had been performed. The outcomes showed that AAV-MYDGF improved endothelial function, decreased endothelial cell apoptosis (Fig. four, A to D), decreased inflammation and adhesion Fc Receptor-like 6 (FCRL6) Proteins medchemexpress molecule expression of MAECs, enhanced IR, and decreased body weight get (fig. S7, A to H), compared with.
