). These fragments could represent nucleotide sequences specific to L. polyphyllus. Other
). These fragments may possibly represent nucleotide sequences particular to L. polyphyllus. Other primers amplified fragments particular for groups of plants (RAPD: OPA-2 arge (about 2000 bp) fragments from the DNA of most plants with the `Minaret’ selection; a 400 bp Hydroxyflutamide custom synthesis fragment particular for Smolensk region plants; QR-5450; and 1500 bp fragments in the `Minaret’ selection; REMAP: MS7Wis00 bp in the feral Biology 2021, 10, x FOR PEER Evaluation six of 18 population at MBG). Such fragments might be of interest as markers for those populations (Figure 1).Figure 1. RAPD profile Figure 1. OPA-2 profile with the OPA-2 primer for L. polyphyllus. (i)–invasive population. [f]–feral with all the RAPD primer for L. polyphyllus. (i)–invasive population, [f]–feral population, population.The genetic diversity in between populations and also the `Minaret’ variety varied from 0.196 The genetic diversity among populations as well as the `Minaret’ wide variety varied from to 0.341 and was 0.260, on Safranin Autophagy average (Table three). 0.196 to 0.341 and was 0.260, on typical (Table three).Table 3. Genetic diversity between the analysed populations of L. polyphyllus calculated according Table 3. Genetic diversity involving of analysed populations of REMAP. to Nei and Li applying the combination themethods RAPD, ISSR andL. polyphyllus calculated based on Nei and Li using the mixture of methods RAPD, ISSR and REMAP. Kostroma Smolensk Moscow Kaluga `Minaret’ Collection Web site MBG (f) Kostroma Smolensk Moscow Kaluga MBG `Minaret’ Range Variety (i) (i) (i) (i) Collection Site(i) (i) 0.000 0.000 0.216 0.000 0.209 0.260 0.216 0.000 0.231 0.258 0.209 0.260 0.269 0.284 0.231 0.258 0.302 0.315 0.269 0.284 `Minaret’ Wide variety 0.302 0.315 (i)–invasive population, (f)–feral population.Kostroma (i) Kostroma (i) Smolensk (i) Moscow (i) Smolensk (i) Kaluga (i) Moscow (i) MBG (f) Kaluga (i) `Minaret’ MBG (f) Assortment(i)(i)(f)0.000 0.196 0.000 0.0.196 0.318 0.198 0.0.000 0.341 0.000 0.230 0.000 0.276 0.341 0.276 0.0.000 0.0.(i)–invasive population, (f)–feral population.Moreover, the genetic diversity inside invasive and feral populations as well as the `Minaret’ range was calculated. To evaluate the genetic diversity within invasiveand the In addition, the genetic diversity within invasive and feral populations population, the populations from Moscow, Kaluga, Smolensk, anddiversity inside invasive pop`Minaret’ selection was calculated. To evaluate the genetic Kostroma regions were pooled (34 samples). The amplified fragment profiles from 34 plant samples wereregions had been ulation, the populations from Moscow, Kaluga, Smolensk, and Kostroma presented as pooled (34 samples). The amplified fragment profiles from 34 plant samples had been presented as binary matrices and utilised to decide genetic diversity values (Supplementary Table S2). According to the technique (RAPD, ISSR, or REMAP), the mean values of genetic diversity varied, however the common trend was the identical: the invasive population hadBiology 2021, ten,6 ofbinary matrices and utilised to ascertain genetic diversity values (Supplementary Table S2). Depending on the approach (RAPD, ISSR, or REMAP), the imply values of genetic diversity varied, but the general trend was precisely the same: the invasive population had the highest values, and the `Minaret’ variety had the lowest values. With all of the techniques combined, the imply Biology 2021, 10, x FOR PEER Assessment 7 of 18 worth of genetic diversity was 0.294 for the invasive population and 0.248 for the feral MBG population, which was 1.2-times reduce than that.
