In G. sinensis beneath Salt Stress as well as the Effect of Exogenou 0.05.Agriculture 2021, 11, x FOR PEER Review Agriculture 2021, 11,11 of 21 10 ofFigure five. Effects of distinctive salt concentrations and addition of exogenous calcium ions on the Figure 5. Effects+of different salt concentrations and addition of exogenous calcium ions around the contents of Na , K+ , and Ca2+ in G. sinensis seedlings. (A ) Na+ , K+ , and Ca2+ content material in differcontents of Na+, K+, and Ca2+ in G. sinensis seedlings. (A ) Na+, K+, and Ca2+ content material in diverse ent DL-AP4 supplier tissue components of G. sinensis at distinct salt concentrations and with diverse concentrations of tissue components of G. sinensis at different salt concentrations and with diverse concentrations of calcalcium CK can be a G. sinensis plant plant in standard hydroponic culture in Hogland Hogland nutrient cium ions.ions. CK is often a G. sinensisgrown grown in typical hydroponic culture in nutrient solusolution. S1 and G. sinensis plants treated with concentration of one hundred of 100 mmol/L NaCl, retion. S1 and S2 areS2 are G. sinensis plants treated with concentrationand 200and 200 mmol/L NaCl, spectively; S1+C1, + C1, S1 + C2, andrepresent represent one hundred mmol/L the additionthe 5, 10, and of five, ten, respectively; S1 S1+C2, and S1+C3 S1 + C3 one hundred mmol/L NaCl with NaCl with of addition 15 mmol/LCaCl2, respectively. The ion content material ion content material was measured and analyzed by inductively and 15 mmol/LCaCl2 , respectively. The was measured and analyzed by inductively coupled plasma optical emission spectrometry just after one week ofone week of plant remedy. Verrucarin A MedChemExpress Theaverage the coupled plasma optical emission spectrometry immediately after plant therapy. The data are the data are SD of three independent replicates. The imply values represented by the distinct letters have been sigaverage SD of three independent replicates. The imply values represented by the distinctive letters nificantly unique in Tukey’s test at p 0.05. have been substantially distinctive in Tukey’s test at p 0.05.three.10. Effects of of Exogenous Calcium on Plant Phenolic Compounds under Salt Tension 3.10. Effects Exogenous Calcium on Plant Phenolic Compounds below Salt Strain Subsequently, we studied the accumulation of phenolic compounds in in unique Subsequently, we studied the accumulation of phenolic compounds various tissues tissues treated with 100 mmol/L NaCl (S1), 100 NaCl + ten mmol/L CaCl (S1 + C2), and treated with one hundred mmol/L NaCl (S1), 100 mmol/L mmol/L NaCl + 10 mmol/L CaClnormal growth state (CK). Visualization of phenolic materials was performed by hierarchical cluster evaluation (Figure six). Twenty-three phenolic compounds and L-phenylalanine had been determined by the LC S technique in methanol extracts of roots, stems, and leavesAgriculture 2021, 11,11 ofof G. sinensis, plus the types of compounds measured for the differently treated samples showed no distinction. It can be observed in the heat map that these compounds have been primarily divided into 3 groups around the basis of their tissue-specific accumulation patterns and participated in salt strain responses to varying degrees. The C6C3-type compound (p-hydroxycinnamic acid, p-coumaric acid), the C6C3C6-type compound (naringenin, glycyrrhizin, genistein, quercetin), and L-phenylalanine have been mainly accumulated inside the roots (Figure 7A). There was a slight improve but not important within the case of salt treatment alone, and a pretty substantial increase in case of exogenous calcium addition. The C6C1type compound (two,5-dihydroxybenzoic acid) and the.
