Round cells. The spermatids appear with round nuclei and dark clumps of heterochromatin and cytoplasm wealthy in mitochondria, with lipid droplets in between the cells (Figure 6a,b). (S)-Mephenytoin medchemexpress Sertoli cells appear with regular nuclei plus the basement membranes enclosed by myoid cells (Figure 6a). The mature spermatozoa were observed with acrosomes at the head, and condensed nuclei, neck, and midpieces with mitochondrial sheaths (Figure 6b).Figure 6. (a,b): Electron micrograph of a section of rat’s testis within the manage group. (a) showing standard seminiferous tubule with normal spermatogenic epithelium (arrow), spermatogonia (Sg), primary spermatocytes (Sc), and spermatids (Sd). Sertoli cell (Se) appeared with all the nucleus (N) and also the basement membrane enclosing myoid cell (my) (000). (b): showing standard sperms. The sperms have flagellum (head arrow) and an elongated condensed nucleus (N) and an acrosomal cap (arrow). The spermatids appeared (Sd) using the nucleus and cytoplasm rich in mitochondria (M), and lipid droplets (L) amongst the cells (500).Electron micrographs of testis of rats treated with EVOO for 15 days showed the standard seminiferous tubules with regular spermatids and typical nuclei. Sperm appeared with elongated condensed nuclei and acrosomal caps at the fronts of the heads. Cephalothin Description vacuoles had been observed inside the cells (Figure 7a). The sperm had standard flagella-elongated condensed nuclei and acrosomal caps. Most sections on the sperm flagella had normal structures, comprising midpieces, principal pieces, and end pieces, with the typical arrangements of flagellar axonemes (Figure 7b).Biology 2021, ten,7 ofFigure 7. (a,b): Electron micrograph of a section of rat’s testis, treated with EVOO for 15 days. (a) showing typical seminiferous tubule with regular spermatids (Sd) and also a typical nucleus. Sperm appeared with an elongated condensed nucleus (N) and an acrosomal cap (arrow) at the top point of the head. Notice vacuoles (v) in cells (000). (b) displaying that the majority of the different parts on the sperm flagellum have normal structure, midpiece (Mp), principal piece (Pp), and finish piece (Ep), together with the common arrangement of flagellar axoneme. Sperms have regular flagellum and elongated condensed nucleus (N) and an acrosomal cap (arrow) (500).Having said that, the testis of rats treated with paracetamol for 15 days showed seminiferous tubules with quite a few adjustments, key spermatocytes with pyknotic nuclei, and ruptured cell membranes. Some sperm had missing flagella with elongated and condensed abnormal nuclei. Moreover, wide separations were observed between neighboring cells and vacuoles for many cells (Figure 8a). Various alterations appeared inside the seminiferous tubules. Sertoli cells appeared, with abnormal chromatin in their nuclei and basement membranes, enclosing myoid cells. The majority of the spermatogonia and main spermatocytes showed condensed chromatin in their nuclei as well as the nuclei in some major spermatocytes were necrotic (Figure 8b).Figure 8. (a,b): Electron micrograph of a section of rat’s testis treated with paracetamol for 15 days. (a) showing seminiferous tubule with various alterations, key spermatocytes (Sc) with pyknotic nucleus (N), along with the rupture from the cell membrane (arrow). Some sperm without having flagellum (head arrow) and elongated condensed abnormal nucleus (N). Notice the wide separation (s) in between neighboring cells and vacuoles (v) in most cells (500). (b) showing seminiferous tubules with many adjustments, Sertoli cell (Se) appeared with abnormal chr.
