In G. sinensis beneath Salt Stress plus the Impact of Exogenou 0.05.Agriculture 2021, 11, x FOR PEER Assessment Agriculture 2021, 11,11 of 21 10 ofFigure 5. Effects of different salt concentrations and addition of exogenous calcium ions on the Figure 5. Effects+of unique salt concentrations and addition of exogenous calcium ions on the contents of Na , K+ , and Ca2+ in G. sinensis seedlings. (A ) Na+ , K+ , and Ca2+ content material in differcontents of Na+, K+, and Ca2+ in G. sinensis seedlings. (A ) Na+, K+, and Ca2+ content material in unique ent tissue components of G. sinensis at distinctive salt concentrations and with various concentrations of tissue components of G. sinensis at unique salt concentrations and with distinctive concentrations of calcalcium CK is a G. sinensis plant plant in standard hydroponic culture in Hogland Hogland nutrient cium ions.ions. CK can be a G. sinensisgrown grown in standard hydroponic culture in nutrient solusolution. S1 and G. sinensis Promestriene manufacturer plants treated with concentration of 100 of one hundred mmol/L NaCl, retion. S1 and S2 areS2 are G. sinensis plants treated with concentrationand 200and 200 mmol/L NaCl, spectively; S1+C1, + C1, S1 + C2, andrepresent represent one hundred mmol/L the additionthe five, ten, and of 5, 10, respectively; S1 S1+C2, and S1+C3 S1 + C3 100 mmol/L NaCl with NaCl with of addition 15 mmol/LCaCl2, respectively. The ion content ion content was measured and analyzed by inductively and 15 mmol/LCaCl2 , respectively. The was measured and analyzed by inductively coupled plasma optical emission spectrometry following one week ofone week of plant treatment. Theaverage the coupled plasma optical emission spectrometry immediately after plant treatment. The data are the data are SD of three independent replicates. The mean values represented by the unique letters were sigaverage SD of 3 independent replicates. The imply values represented by the various letters nificantly various in Tukey’s test at p 0.05. have been considerably distinct in Tukey’s test at p 0.05.three.ten. Effects of of Exogenous Calcium on Plant Phenolic Compounds under Salt Anxiety 3.ten. Effects Exogenous Calcium on Plant Phenolic Compounds below Salt Strain Subsequently, we studied the accumulation of phenolic compounds in in distinctive Subsequently, we studied the accumulation of phenolic compounds distinct tissues tissues treated with 100 mmol/L NaCl (S1), 100 NaCl + 10 mmol/L CaCl (S1 + C2), and treated with 100 mmol/L NaCl (S1), one hundred mmol/L mmol/L NaCl + 10 mmol/L CaClnormal growth state (CK). Visualization of phenolic materials was performed by hierarchical cluster evaluation (Figure six). Twenty-three phenolic compounds and L-phenylalanine were determined by the LC S method in methanol extracts of roots, stems, and leavesAgriculture 2021, 11,11 ofof G. sinensis, plus the forms of compounds measured for the differently treated samples showed no distinction. It might be seen in the heat map that these compounds have been Benzimidazole supplier mostly divided into 3 groups on the basis of their tissue-specific accumulation patterns and participated in salt tension responses to varying degrees. The C6C3-type compound (p-hydroxycinnamic acid, p-coumaric acid), the C6C3C6-type compound (naringenin, glycyrrhizin, genistein, quercetin), and L-phenylalanine have been mostly accumulated within the roots (Figure 7A). There was a slight raise but not important in the case of salt remedy alone, plus a incredibly considerable enhance in case of exogenous calcium addition. The C6C1type compound (two,5-dihydroxybenzoic acid) and also the.
