Stained with hematoxylin and eosin (H E dye) for light microscopy examination. For the transmission electron microscopy, compact pieces of testis were promptly fixed in 4F1G in phosphate buffer (pH 7.2) for three h at four C, then post-fixed in 2 OsO4 in the same buffer at 4 C for 1 h. The specimens had been dehydrated via a graded series of ethanol, embedded in an Epon-Araldite mixture, and polymerized at 60 C. Ultrathin sections (50 nm) from chosen areas had been reduce with glass knives on an LKB ultramicrotome double-stained with uranyl acetate and lead citrate, and examined using a Jeol 100CX electron microscope. Table 1 shows the morphological parameters inside the cell study.Table 1. Morphological parameters thought of in the cell study. Cell Portion Nucleus Cytoplasm Plasma membrane Flagella Morphological Parameters Shape, Chromatin, Variety of nucleoli Morphology of organelles, Vacuoles, Lipid droplets Shape, Basement membrane Shape2.five. Statistical Analysis The information had been presented because the mean SD of ten replicates and had been analyzed by a one-way ANOVA and LSD post hoc tests Benoxinate hydrochloride Technical Information applying SPSS application. The outcomes were deemed statistically substantial when p 0.05.Biology 2021, 10,5 of3. Results 3.1. Histological Results Light microscopy examination with the testis sections of the control rats showed the typical functions of regular seminiferous tubules, with regular spermatogenic cells, Sertoli cells, and spermatozoa (Figure two). The testicular tissue of rats given EVOO for 15 days showed no clear adjustments in Cefapirin sodium MedChemExpress comparison to the control group. The seminiferous tubules appeared with typical spermatogenic cells, sperm, and Sertoli cells (Figure 3).Figure 2. Section of testis in manage group rats displaying normal structure of seminiferous tubules with regular germinal epithelium (GE), Sertoli cells (arrow), and sperm (S) (00).Figure 3. Section of testis of rats treated with EVOO for 15 days displaying regular seminiferous tubules with standard germinal epithelium, Sertoli cells (arrow) (GE), and sperm (S) (00).The testis sections of animals provided paracetamol for 15 days showed testicular distortion in comparison to the controls. Loss of the regular testicular structure, with markedly disorganized spermatogenic cysts with separated and ruptured basement membranes of the germinal epithelial cells, and degenerated germ cells with pyknotic nuclei, are clearly observed (Figure 4). In addition, the testis sections from the rats treated with EVOO and paracetamol for 15 days showed improvement in most seminiferous tubules and much less prominent histopathological alterations in comparison to the paracetamol group (Figure five).Figure four. Section of testis of rats treated with paracetamol for 15 days showing disorganized arrangement of spermatogenic cysts (arrows), separated and ruptured basement membrane in the germinal epithelial cells (head arrows), degenerated germ cells with pyknotic nuclei (P) (H E 00).Biology 2021, ten,six ofFigure five. Section of testis of rats treated with EVOO and paracetamol for 15 days displaying most seminiferous tubules with regular structure (arrow) (00).three.two. Electron Microscopy Final results Electron micrographs of your testis in the control rats show the standard structure of seminiferous tubules. They are lined with spermatogenic epithelial cells, followed by the usual sequence of spermatogonia, major spermatocytes, and spermatids. Spermatogenic cells seem with standard nuclei containing peripheral clumped chromatin. The primary spermatocytes seem above the spermatogonia as massive.
