Etermining the resistive index only in kidneys from deceased donors. In summary, a renal arterial resistive index higher than 0.66 may determine chronic kidney disease stage 4 or higher in patients with renal allograft.Author ContributionsConceived and designed the experiments: SOW MT. Performed the experiments: SOW HCT LNP MC HA MT. Analyzed the data: SOW HCT LNP MC HA MT. Contributed ML240 reagents/materials/analysis tools: SOW HCT LNP MC HA MT. Wrote the paper: SOW HCT LNP MC HA MT.
Treatment strategies for high-grade primary brain tumors such as glioblastoma multiforme (GBM) have failed to significantly and consistently extended survival despite 50 years of advances in radiotherapy, chemotherapy, and surgical techniques [1]. Immunotherapy remains an attractive option, although classical approaches that have shown some promise in other malignancies have generally been disappointing when applied to GBM [2?]. A variety of immune cell therapy approaches to GBM have been attempted over the past several years. Ex vivo culture of cytotoxic T lymphocytes (CTL) from tumor-draining lymph nodes [8,9], tumor-infiltrating lymphocytes (TIL), and HLA-mismatched T cells from Pleuromutilin healthy donors with systemic and intracranial infusion have all met with limited success. The most predominant cell therapy consisted of autologous lymphokine-activated killer (LAK) cells, a combination of NK and T lymphocytes cultured in high doses of IL-2. Although promising in early studies, these therapies fall short for several reasons. CTL therapies are based on adaptive immunity (i.e. MHC-restricted, antigen-specific responses) and aretherefore dependent upon the dose of T cell clones that specifically recognize various tumor-associated peptide antigens dispersed among various subsets of glioma cells. Infusion or intracranial placement of HLA-mismatched CTL relies on allogeneic recognition of transplantation antigens and is highly dependent on glioma cell MHC Class I expression [10,11]. LAK cell preparations are difficult to consistently manufacture, are short-lived in vivo [12], and are complicated by IL-2 related toxicity once infused or placed in the tumor resection cavity [2,13?6]. To overcome these issues, during the past six years, we developed a robust method for generating anti-glioma immunocompetent cd T cells. We have shown that ex vivo expanded/ activated cd T cells from healthy volunteers are cytotoxic to highgrade gliomas in both in vitro and in specific in vivo models designed to replicate therapeutic conditions [17?9]. The anti-tumor cytotoxicity of cd T cells is at least partially due to innate recognition of stress-induced NKG2D ligands such as MICA/B and UL-16 binding proteins (ULBP) that are expressed on GBM but not on adjacent normal brain tissue [17,20,21].Drug Resistant cd T Cell ImmunotherapyOne of the most formidable obstacles in the treatment of cancer has been chemotherapy-induced hematopoietic cell toxicity and the associated loss of an effective and robust immune response [22]. To circumvent these consequences, concurrent with the development of immunocompetent cell expansion methods, we developed a gene therapy-based strategy whereby anti-cancer immune cells are genetically engineered to resist the toxic effects of chemotherapy drugs, which allows for the combined administration of 18325633 chemotherapy and immunotherapy. This drug resistant immunotherapy (or DRI) approach has been shown to be effective in animal models of sarcoma and neuroblastoma. [23?5].Etermining the resistive index only in kidneys from deceased donors. In summary, a renal arterial resistive index higher than 0.66 may determine chronic kidney disease stage 4 or higher in patients with renal allograft.Author ContributionsConceived and designed the experiments: SOW MT. Performed the experiments: SOW HCT LNP MC HA MT. Analyzed the data: SOW HCT LNP MC HA MT. Contributed reagents/materials/analysis tools: SOW HCT LNP MC HA MT. Wrote the paper: SOW HCT LNP MC HA MT.
Treatment strategies for high-grade primary brain tumors such as glioblastoma multiforme (GBM) have failed to significantly and consistently extended survival despite 50 years of advances in radiotherapy, chemotherapy, and surgical techniques [1]. Immunotherapy remains an attractive option, although classical approaches that have shown some promise in other malignancies have generally been disappointing when applied to GBM [2?]. A variety of immune cell therapy approaches to GBM have been attempted over the past several years. Ex vivo culture of cytotoxic T lymphocytes (CTL) from tumor-draining lymph nodes [8,9], tumor-infiltrating lymphocytes (TIL), and HLA-mismatched T cells from healthy donors with systemic and intracranial infusion have all met with limited success. The most predominant cell therapy consisted of autologous lymphokine-activated killer (LAK) cells, a combination of NK and T lymphocytes cultured in high doses of IL-2. Although promising in early studies, these therapies fall short for several reasons. CTL therapies are based on adaptive immunity (i.e. MHC-restricted, antigen-specific responses) and aretherefore dependent upon the dose of T cell clones that specifically recognize various tumor-associated peptide antigens dispersed among various subsets of glioma cells. Infusion or intracranial placement of HLA-mismatched CTL relies on allogeneic recognition of transplantation antigens and is highly dependent on glioma cell MHC Class I expression [10,11]. LAK cell preparations are difficult to consistently manufacture, are short-lived in vivo [12], and are complicated by IL-2 related toxicity once infused or placed in the tumor resection cavity [2,13?6]. To overcome these issues, during the past six years, we developed a robust method for generating anti-glioma immunocompetent cd T cells. We have shown that ex vivo expanded/ activated cd T cells from healthy volunteers are cytotoxic to highgrade gliomas in both in vitro and in specific in vivo models designed to replicate therapeutic conditions [17?9]. The anti-tumor cytotoxicity of cd T cells is at least partially due to innate recognition of stress-induced NKG2D ligands such as MICA/B and UL-16 binding proteins (ULBP) that are expressed on GBM but not on adjacent normal brain tissue [17,20,21].Drug Resistant cd T Cell ImmunotherapyOne of the most formidable obstacles in the treatment of cancer has been chemotherapy-induced hematopoietic cell toxicity and the associated loss of an effective and robust immune response [22]. To circumvent these consequences, concurrent with the development of immunocompetent cell expansion methods, we developed a gene therapy-based strategy whereby anti-cancer immune cells are genetically engineered to resist the toxic effects of chemotherapy drugs, which allows for the combined administration of 18325633 chemotherapy and immunotherapy. This drug resistant immunotherapy (or DRI) approach has been shown to be effective in animal models of sarcoma and neuroblastoma. [23?5].
