Cer diseases. The meta-analysis including our present study was further conducted, which denied the meaningful association between them (Figure 3). We speculated that some preventive effects of coffee intake might outweigh the risks of increased gastric acid secretion: relaxing effect, antioxidant effect, phytochemical effect, and so on [46?8]. For GERD, we also could not detect a significant association between coffee intake and the incidence of GERD (both RE and NERD), although some past study have reported that coffee intake may predispose to GERD syndrome [19]. Besides the stimulating effect upon gastric acrid production, it was also reported that coffee intake relaxes the lower esophageal sphincter [49], which might cause the chronic gastric acid reflux. Excessive secretion of gastric acid can damage not only the gastroduodenal but also esophageal mucosa, but our multivariate analysis of the healthy subjects (Table 16574785 3) did not detect significant association between coffee Title Loaded From File consumption and GERD (both RE and NERD). At present, epidemiological studies concerning coffee intake and GERD have been very few. Many studies like ours should be accumulated in the future, which will make it possible to perform the reliable meta-analysis. One of limitations of our present study was of course a crosssectional design, which should be precisely validated in the future prospective study. We are following the present large-scale cohort to validate our present conclusion in the upcoming trial. Anotherlimitation of our study was lacking more detailed information of coffee, such as kinds of coffee beans, use of milk or sugar, regular coffee or not, the time of coffee drinking, etc. These minute data concerning coffee intake will be added to the future study, which will make our next research more accurate and polished to verify our present conclusion.Supporting InformationFigure S1 Flow diagram of the meta-analysis literature search results. (DOC) Table S1 Summary characteristics of cohort or casecontrol studies were included from the meta-analysis that compare the relationship of coffee and peptic ulcer. (XLS) Table S2 Summary characteristics of cohort or casecontrol studies were excluded from the meta-analysis that compare the association of coffee and peptic ulcer. (XLS) Document S1 References used in Table S1 S2.(DOC)AcknowledgmentsWe thank Mr. Minoru Okada, Mr. Masanori Fujiwara, and Mr. Koichi Yamashita (Kameda Medical Center Makuhari, Chiba-shi, Chiba, Japan) for great assistance with establishment and maintenance of the study database.Author ContributionsConceived and designed the experiments: TS NY. Analyzed the data: TS. Wrote the paper: TS NY. Acquisition of the data: NY TM. Critical revision of the manuscript for important intellectual content: SK YT MF MO TM KK. Guarantor of the study: KK.
Microbial insecticides containing d-endotoxins (Cry proteins) from Bacillus thuringiensis (Bt) have been used as an alternative to conventional chemical pesticides in agriculture for almost 60 years and recently as resource for insect-resistant genetically modified (GM) plants [1]. Currently, more than 90 of the feedstuffs for pigs 23977191 contain genetic modified compounds [2] and the interest in GM crops is continuously increased because of higher agronomic productivity and more nutritious food without the use of pesticides. Since the introduction of GM crops many feeding trials focussed on issues related to Title Loaded From File consumer safety have been conducted in various anima.Cer diseases. The meta-analysis including our present study was further conducted, which denied the meaningful association between them (Figure 3). We speculated that some preventive effects of coffee intake might outweigh the risks of increased gastric acid secretion: relaxing effect, antioxidant effect, phytochemical effect, and so on [46?8]. For GERD, we also could not detect a significant association between coffee intake and the incidence of GERD (both RE and NERD), although some past study have reported that coffee intake may predispose to GERD syndrome [19]. Besides the stimulating effect upon gastric acrid production, it was also reported that coffee intake relaxes the lower esophageal sphincter [49], which might cause the chronic gastric acid reflux. Excessive secretion of gastric acid can damage not only the gastroduodenal but also esophageal mucosa, but our multivariate analysis of the healthy subjects (Table 16574785 3) did not detect significant association between coffee consumption and GERD (both RE and NERD). At present, epidemiological studies concerning coffee intake and GERD have been very few. Many studies like ours should be accumulated in the future, which will make it possible to perform the reliable meta-analysis. One of limitations of our present study was of course a crosssectional design, which should be precisely validated in the future prospective study. We are following the present large-scale cohort to validate our present conclusion in the upcoming trial. Anotherlimitation of our study was lacking more detailed information of coffee, such as kinds of coffee beans, use of milk or sugar, regular coffee or not, the time of coffee drinking, etc. These minute data concerning coffee intake will be added to the future study, which will make our next research more accurate and polished to verify our present conclusion.Supporting InformationFigure S1 Flow diagram of the meta-analysis literature search results. (DOC) Table S1 Summary characteristics of cohort or casecontrol studies were included from the meta-analysis that compare the relationship of coffee and peptic ulcer. (XLS) Table S2 Summary characteristics of cohort or casecontrol studies were excluded from the meta-analysis that compare the association of coffee and peptic ulcer. (XLS) Document S1 References used in Table S1 S2.(DOC)AcknowledgmentsWe thank Mr. Minoru Okada, Mr. Masanori Fujiwara, and Mr. Koichi Yamashita (Kameda Medical Center Makuhari, Chiba-shi, Chiba, Japan) for great assistance with establishment and maintenance of the study database.Author ContributionsConceived and designed the experiments: TS NY. Analyzed the data: TS. Wrote the paper: TS NY. Acquisition of the data: NY TM. Critical revision of the manuscript for important intellectual content: SK YT MF MO TM KK. Guarantor of the study: KK.
Microbial insecticides containing d-endotoxins (Cry proteins) from Bacillus thuringiensis (Bt) have been used as an alternative to conventional chemical pesticides in agriculture for almost 60 years and recently as resource for insect-resistant genetically modified (GM) plants [1]. Currently, more than 90 of the feedstuffs for pigs 23977191 contain genetic modified compounds [2] and the interest in GM crops is continuously increased because of higher agronomic productivity and more nutritious food without the use of pesticides. Since the introduction of GM crops many feeding trials focussed on issues related to consumer safety have been conducted in various anima.
