Ay explain this result: Either the LIMKI-3 supplier transmission of a given variant is a stochastic process accompanied by mechanisms that prevent the transmission/amplification of other viruses, or transmitted viruses have specific traits to overcome the multiple “gatekeepers” of the vaginal mucosa. The recent isolation and cloning of T/F virus envelopes and fulllength infectious clones enables the study of their properties under controlled conditions. We used recently 3PO described [4] isogenic, replication-competent proviral constructs in which the env sequences encoding the ectodomain (gp120 and ectodomain of gp41) 22948146 of the Env glycoporotein were derived from either T/F HIV-1 variants or chronic/reference (C/R) HIV-1 strains utilized as control viruses. We studied transmission of these viruses in a recently developed system [5] of collagen raft-supported blocks of human cervical tissue. To investigate the abilities of several HIV-1 strains toinitiate infection in human cervical tissue ex vivo, we investigated the efficiencies of viral replication, the cellular targets of these viruses, and the target cell activation status.Materials and Methods HIV-1 Virus StrainsWe recently described a molecular approach to express env sequences of interest in cis in isogenic, replication-competent, NL43-based backbones infectious molecular clones (Env-IMC), refered here as “NL-Env.ecto”. To avoid creating chimerism in any reading frames overlapping env, heterologous env sequences encoding the ectodomain were cloned into the NL4-3 backbone, wherein the recombinant viruses express full-length Env [4]. We used three R5-tropic reference HIV-1 variant (NL-SF162.ecto, NL-YU-2.ecto, and NL-BaL.ecto), as well as eight viruses encoding CCR5-utilizing env genes from clade B mucosally transmitted T/F HIV-1 variants, including NL-CH077.ecto, NL-1051.TD12.ecto, NL-1051.C22.ecto, NL-TT31P.2G1.ecto, NL-TT31P.2F10.ecto, NL-SC22.3C2.ecto, NL-RHPA.ecto, and NL-9010.A1.ecto, with env genes derived, respectively, from subjects 700010077 (male; single variant transmission); 1051-12 (female; infected with two related variants); TT31P (female; infected with two related variants); SC22 23727046 (female; single variant transmission), RHPA4259 (female; single variant transmission); and 9010 (female; single variant transmission) as described by [2]).Transmission of Founder HIV-1 to Cervical ExplantsIn parallel, we employed the full-length IMC, CH077.t and RHPA.c, which represent the T/F viruses from subjects 700010077 and RHPA4259, respectively [6]. We have previously established that the cellular tropism of Env-IMC closely match that of their respective matched full-length IMC or isolates [6]. The R5-tropic HIV-1BaL (NIH AIDS Research Reference Reagent Program, catalogue #510), isolated from a chronically infected human infant lung, served as another control virus. Virus stocks were prepared essentially as described [4,6]. Briefly, 293T cells were 
transfected with proviral DNA, medium was changed at 16 hours, and virus stocks harvested at 60 hours. HIV1 BaL was grown in PBMC. All stocks were titered on TZM-bl cells, and infectious units (IU) per ml were determined by betagalactosidase staining for quality assurance. Viral stocks were directly used for inoculation of tissues. TCID50 on TZM-bl cells of the different viruses varied from 1×107 to 4.5×107 (for the C/R HIV-1 variants the range was from 2.5 to 4.0 x107 and for T/F HIV-1 variants it was from 1.0 to 4.5×107). Such differences in TCID50 valu.Ay explain this result: Either the transmission of a given variant is a stochastic process accompanied by mechanisms that prevent the transmission/amplification of other viruses, or transmitted viruses have specific traits to overcome the multiple “gatekeepers” of the vaginal mucosa. The recent isolation and cloning of T/F virus envelopes and fulllength infectious clones enables the study of their properties under controlled conditions. We used recently described [4] isogenic, replication-competent proviral constructs in which the env sequences encoding the ectodomain (gp120 and ectodomain of gp41) 22948146 of the Env glycoporotein were derived from either T/F HIV-1 variants or chronic/reference (C/R) HIV-1 strains utilized as control viruses. We studied transmission of these viruses in a recently developed system [5] of collagen raft-supported blocks of human cervical tissue. To investigate the abilities of several HIV-1 strains toinitiate infection in human cervical tissue ex vivo, we investigated the efficiencies of viral replication, the cellular targets of these viruses, and the target cell activation status.Materials and Methods HIV-1 Virus StrainsWe recently described a molecular approach to express env sequences of interest in cis in isogenic, replication-competent, NL43-based backbones infectious molecular clones (Env-IMC), refered here as “NL-Env.ecto”. To avoid creating chimerism 
in any reading frames overlapping env, heterologous env sequences encoding the ectodomain were cloned into the NL4-3 backbone, wherein the recombinant viruses express full-length Env [4]. We used three R5-tropic reference HIV-1 variant (NL-SF162.ecto, NL-YU-2.ecto, and NL-BaL.ecto), as well as eight viruses encoding CCR5-utilizing env genes from clade B mucosally transmitted T/F HIV-1 variants, including NL-CH077.ecto, NL-1051.TD12.ecto, NL-1051.C22.ecto, NL-TT31P.2G1.ecto, NL-TT31P.2F10.ecto, NL-SC22.3C2.ecto, NL-RHPA.ecto, and NL-9010.A1.ecto, with env genes derived, respectively, from subjects 700010077 (male; single variant transmission); 1051-12 (female; infected with two related variants); TT31P (female; infected with two related variants); SC22 23727046 (female; single variant transmission), RHPA4259 (female; single variant transmission); and 9010 (female; single variant transmission) as described by [2]).Transmission of Founder HIV-1 to Cervical ExplantsIn parallel, we employed the full-length IMC, CH077.t and RHPA.c, which represent the T/F viruses from subjects 700010077 and RHPA4259, respectively [6]. We have previously established that the cellular tropism of Env-IMC closely match that of their respective matched full-length IMC or isolates [6]. The R5-tropic HIV-1BaL (NIH AIDS Research Reference Reagent Program, catalogue #510), isolated from a chronically infected human infant lung, served as another control virus. Virus stocks were prepared essentially as described [4,6]. Briefly, 293T cells were transfected with proviral DNA, medium was changed at 16 hours, and virus stocks harvested at 60 hours. HIV1 BaL was grown in PBMC. All stocks were titered on TZM-bl cells, and infectious units (IU) per ml were determined by betagalactosidase staining for quality assurance. Viral stocks were directly used for inoculation of tissues. TCID50 on TZM-bl cells of the different viruses varied from 1×107 to 4.5×107 (for the C/R HIV-1 variants the range was from 2.5 to 4.0 x107 and for T/F HIV-1 variants it was from 1.0 to 4.5×107). Such differences in TCID50 valu.
