Cells in the lungs of mice.Difference in 1516647 Time-course Kinetics of Type-I Interferon Amount in 
Bronchoalveolar Lavage Fluid in Lethally and Non-lethally Infected MiceIn the above study, it was shown that FasL mRNA expression in the lung of lethally infected mice was detected at earlier than in non-lethally infected mice (Fig. 3A and C). To clarify the detail of the differences in non-lethal or lethal infected conditions, the time dependent kinetics of production of type-I interferon in the lungs of mice infected non-lethally and lethally were evaluated. The amounts of type-I interferon in the broncho alveolar lavage uid (BALF) in the lungs of these mice were assessed. Murine IFN-b specific ELISA showed that production of IFN-b protein in the BALF of mice infected with a lethal titer of the PR/8 virus was induced at 3DPI and this production level was slightly decreased at 5DPI (Fig. 5). In the case of non-lethal infection, IFN-b production was not detected in the BALF at 3DPI, but was slightly detected at 5DPI (Fig. 5). These findings indicate that the time dependent kinetics of IFNb production is different between the lethal and non-lethal infections of the virus in the lungs of mice.Importance of Type I IFN and FasL in InfluenzaFigure 5. Production of IFN- ?in the lungs of mice infected lethally or non-lethally with the PR/8 virus. B6 mice were intranasally infected with 105 (closed triangle) or 102 (open square) pfu/head of the PR/8 virus. At 0, 3 or 5 DPI, the BALF of these mice were isolated. The amount of IFN- ?or total protein contained in these samples was assessed by mouse IFN- ?specific ELISA or BCA protein assay, respectively. The amounts of IFN- ?were normalized by that of the total protein in each sample. “N.D.” means not detected. doi:10.1371/journal.pone.0055321.gDiscussionIn this study, we proposed that type-I IFN production highly induces the expression of FasL on several cells in the lung which leads to the reduction of the survival rate after a lethal infection of PR/8 virus. Previously, it was reported that intranasal administration of anti-Fas specific agonistic antibody induces acute lung inflammation [7,8]. We also found that functional mutation of the FasL gene protects mice from a lethal influenza A virus infection (Fig. 1A) as well as in a previous study [6]. Our data and the previous reports suggest that FasL MedChemExpress 4-IBP mediated signal in lung has a negative effect for protecting host against PR/8 virus infection. Since the same perspective was provided by the assay using the administration of a recombinant chimeric protein inhibitor for FasL/Fas interaction (Fig. 1B), this effect was not due to the other effects mediated by gld/gld mutation or genetic background before the viral infection. In Fig. 2, it is demonstrated that the severity of illness, such as reduction of body weight and survival rate, after influenza A virus infection should get K162 correlate with the initial infected titer of the virus but not the 
titer of the propagated virus in the lung. In this situation, it was shown that induction of FasL gene in lung of mice lethally infected with PR/8 virus was detected earlier than in that of non-lethally infected mice, and this time-course kinetics seemed to correlate with loss of body weight (compared with Fig. 3A versus 3E, and Fig. 3C versus 3F). In addition, it was reported that activation of the Fas signal causes severe inflammation in the lungs of mice [7,8]. Although the series of immunological or pathological rea.Cells in the lungs of mice.Difference in 1516647 Time-course Kinetics of Type-I Interferon Amount in Bronchoalveolar Lavage Fluid in Lethally and Non-lethally Infected MiceIn the above study, it was shown that FasL mRNA expression in the lung of lethally infected mice was detected at earlier than in non-lethally infected mice (Fig. 3A and C). To clarify the detail of the differences in non-lethal or lethal infected conditions, the time dependent kinetics of production of type-I interferon in the lungs of mice infected non-lethally and lethally were evaluated. The amounts of type-I interferon in the broncho alveolar lavage uid (BALF) in the lungs of these mice were assessed. Murine IFN-b specific ELISA showed that production of IFN-b protein in the BALF of mice infected with a lethal titer of the PR/8 virus was induced at 3DPI and this production level was slightly decreased at 5DPI (Fig. 5). In the case of non-lethal infection, IFN-b production was not detected in the BALF at 3DPI, but was slightly detected at 5DPI (Fig. 5). These findings indicate that the time dependent kinetics of IFNb production is different between the lethal and non-lethal infections of the virus in the lungs of mice.Importance of Type I IFN and FasL in InfluenzaFigure 5. Production of IFN- ?in the lungs of mice infected lethally or non-lethally with the PR/8 virus. B6 mice were intranasally infected with 105 (closed triangle) or 102 (open square) pfu/head of the PR/8 virus. At 0, 3 or 5 DPI, the BALF of these mice were isolated. The amount of IFN- ?or total protein contained in these samples was assessed by mouse IFN- ?specific ELISA or BCA protein assay, respectively. The amounts of IFN- ?were normalized by that of the total protein in each sample. “N.D.” means not detected. doi:10.1371/journal.pone.0055321.gDiscussionIn this study, we proposed that type-I IFN production highly induces the expression of FasL on several cells in the lung which leads to the reduction of the survival rate after a lethal infection of PR/8 virus. Previously, it was reported that intranasal administration of anti-Fas specific agonistic antibody induces acute lung inflammation [7,8]. We also found that functional mutation of the FasL gene protects mice from a lethal influenza A virus infection (Fig. 1A) as well as in a previous study [6]. Our data and the previous reports suggest that FasL mediated signal in lung has a negative effect for protecting host against PR/8 virus infection. Since the same perspective was provided by the assay using the administration of a recombinant chimeric protein inhibitor for FasL/Fas interaction (Fig. 1B), this effect was not due to the other effects mediated by gld/gld mutation or genetic background before the viral infection. In Fig. 2, it is demonstrated that the severity of illness, such as reduction of body weight and survival rate, after influenza A virus infection should correlate with the initial infected titer of the virus but not the titer of the propagated virus in the lung. In this situation, it was shown that induction of FasL gene in lung of mice lethally infected with PR/8 virus was detected earlier than in that of non-lethally infected mice, and this time-course kinetics seemed to correlate with loss of body weight (compared with Fig. 3A versus 3E, and Fig. 3C versus 3F). In addition, it was reported that activation of the Fas signal causes severe inflammation in the lungs of mice [7,8]. Although the series of immunological or pathological rea.
